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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRY
The concentration of Mouse PⅠNP in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human CASP7 Antibody is added and binds to CASP7 in the sample
The concentration of Mouse COL4 in the samples is then determined by comparing the OD of the samples to the standard curve
Only those wells that contain Mouse FETUA
Human FLOT2 (Flotillin-2) ELISA Kit Intestinal Lactoferrin Receptor Standards or samples are addedThe Human (FLOT2) Flotillin 2 ELISA Kit measures Flotillin 2 in samples. The plate has been pre coated with Human FLOT2 antibody. FLOT2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FLOT2 Antibody is added and binds to FLOT2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FLOT2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
