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The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of Osteopontin in the specimen
Then a biotinylated detection antibody specific for Mouse POSTN/OSF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Rat TF
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD27BP
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IL6
APOO (Apolipoprotein O) ELISA Kit (Small Sample) MIP1A The absorbance values are measuredThe APOO (Apolipoprotein O) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human APOO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human APOO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
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