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which can be directly detected by electrophoresis after the reaction is completed
The concentration of Human TNF-alpha in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human I-TAC/CXCL11
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG are added to the micro ELISA plate wells
Universal 5-HIAA in the sample or standard competes with a fixed amount of Universal 5-HIAA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 5-HIAA
Collagen IV (5C9) Mouse mAb OCIF which can be directly detectedCollagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
